Karolinska Institutet

Karolinska Institutet, Department of Mardine

The Integrated Cardio Metabolic Centre (ICMC)
, which has been established since 2015 as a research collaboration between Karolinska Institutet and the pharmaceutical company AstraZeneca, addresses a variety of strategic research areas in the fields of cardiovascular and metabolic diseases. It creates aunique interface between academia and industry, provides outstanding infrastructure, and aims to develop innovative therapeutic approaches. ICMC is a unit within the Department of Medicine, Huddinge at the Karolinska Institutet at the Karolinska University Hospital.

We are hereby announcing a 5 month substitute position as Research Engineer, with possibility of extension.

The Group
The working group is made up of a mix of complementary skills in order to quickly and effectively drive the issues forward. We are a team of several postdoctoral researchers working together in a research Group where we identify cells though their transcriptome. Every postdoc has a different tissue focus, including vasculature, kidney, liver, heart and pancreas.

The post of the Research Engineer includes the following tasks:

  1. General day-to-day management of a research group where a lot of projects are running in parallel, i.e. introducing new users to the specialized equipment, data sheet access management, reagent ordering and sample tracking.
  2. Preparation of 384-well plates containing RNA-lysis buffer. We are looking for a person who is very skilled at handling low volume (nanoliter range) dispensing using a Formulatrix Mantis and an Integra ViaFlo
  3. Handling of single cell RNA extracts in 384-well plates and organizing the acquisition, tracking and progress of a large number of plates.
  4. Conversion of RNA to cDNA using automated liquid handling robots (Agilent Bravo) and PCR reactions. Enzymatic fragmentation and tagging of cDNA fragments for Illumina sequencing (HiSeq3000).
  5. Magnetic bead-based nucleotide isolations in 384 well plates, both manually and automated using the liquid handling robots described in the previous Point.
  6. Quality control on cDNA conversion and sequencing library preparation using automatic microfluidic gel electrophoresis (Agilent Bioanalyzer).
  7. Clustering of cDNA libraries on an Illumina cBot2 for sequencing on an Illumina HiSeq3000.

Experience and competence
We are looking for an experienced person with extensive technical knowledge in the field of highthroughput liquid handling. The applicant should have previous experience with liquid handlers and should be proficient in working with sub-microliter volumes. Prior knowledge with sequencers or sequencing data is a merit. A large portion of the work will involve handling of many projects in a very dynamic laboratory, so skills in laboratory and project management are of large merit.

We are looking for a Research Engineer who is able to work dynamically at ICMC, an international and multidisciplinary research institution. Excellent knowledge of the English language is of utmost importance. Personal suitability will be a strong selection criterion. The applicant will be assessed on basis of technical competence, as well as past experience.

Application process
An employment application must contain the following documents in English or Swedish:

1. A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
2. A complete list of publications
3. A summary of current work (no more than one page)
4. A Personal letter

The application is to be submitted through the Varbi recruitment system.

Type of employment Visstidsanställning 3-6 månader
Contract type Full time
City Stockholm
County Stockholms län
Country Sweden
Reference number 2-4791/2018
  • Michael Vanlandewijck‎, michael.vanlandewijck@ki.se
Union representative
  • Yvonne Edlund OFR/ST , yvonne.edlund@ki.se
  • Mats Rudling, SACO, mats.rudling@ki.se
Published 08.Sep.2018
Last application date 17.Sep.2018 11:59 PM CET

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