Karolinska Institutet, Department of Women's and Children's Health

The Department is organized in 10 divisions: Obstetrics and Gynecology, Pediatrics, Pediatric Endocrinology, Pediatric Surgery, Neuropediatrics, Pediatric Oncology, Neonatology, Reproductive Endocrinology, Neuropsychiatry (KIND), Reproductive and Perinatal Health. The divisions have separate scientific profiles and vary in size and have several research groups, dealing with both basic and clinical science.

Research Group
The Petter Brodin research group located at Science for Life Laboratory in Stockholm and affiliated with the unit of clinical pediatrics, KBH, Karolinska Institutet, develops experimental and computational research methods for systems-level analyses of human immune cells. The aim of the group is to understand the regulation and development of human immune systems in general, and the immune system of children in particular.

The lab engineer duties
The current project will be focused on the development of new blood collection and stabilization devices in order to simplify and improve clinical blood sampling and analyses methodologies and allow more sampling previously not possible. The technology will involve the design and manufacturing of microfluidic devices and the detailed testing and development thereof for separation of plasma from cells and in the future immune cell analyses performed at these chips.

Another important focus of the project is the development of novel experimental technologies for single-cell analyses based on droplet barcoding, single-cell dispension in nanowells and the combined analyses of proteins, DNA and mRNA from individual immune cells. The use of free-DNA barcodes will also be a part of the Project.

Skills and personal qualities
The successful candidate must have a PhD in the physical or life sciences. A successful candidate will be thriving in interdisciplinary environments, able and comfortable working independently, but in regular communication with the team leader and other group members and collaborators and meeting deadlines. The candidate must have excellent writing and communication skills in English. Strong and documented expertise in the fabrication of microfluidic devices is essential. Experience in developments of microfluidic biosensors, blood separation devices, the use of BioMEMS and device design using solid works is essential. Experiences with fabrication techniques especially those applicable for large-scale production such as the use of laminate materials, laser and milling techniques, injection molding and general rapid prototyping skills will be beneficial. Knowledge about fluidic device surface chemistry and work with biological fluids, especially blood is important. Finally, the experience of working with free-DNA barcodes and bead-based barcoding of single cells as well as coupling and use of DNA-coupled antibodies and other proteins is an important and valuable aspect of a successful candidate.

Assessment criteria
A selection will be made among qualified applicants on the basis of the following criteria:

  • Documented subject knowledge and experience in relation to the listed qualities above
  • Expressed interest in the project
  • Other documented experience relevant to the subject and planned project

The qualifications of the applicants will be evaluated on an overall basis.

Application process
An application must contain the following documents in English:

  • A personal letter and curriculum vitae
  • A copy of degree certificates and associated certificates
  • A copy of degree projects and any previous publications

The application is to be submitted on the MyNetwork recruitment system.

At Karolinska Institutet a 6-month trial period is required for all permanent appointments.

Type of employment Tillsvidareanställning
Contract type Full time
City Solna
County Stockholms län
Country Sweden
Reference number 2-2997/2018
Contact
  • Petter Brodin, Associate Professor, +46 8 524 813 96
Union representative
  • Lars Ährlund-Richter, SACO, 08 - 5177 34 50, lars.ahrlund@ki.se
  • Carina Eklund, OFR, 08-585 826 65, carina.eklund@ki.se
  • Henry Wölling, SEKO, henry.wolling@ki.se
Published 25.May.2018
Last application date 15.Jun.2018 11:59 PM CET

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