Karolinska Institutet, Dept. of Oncology-Patology, Helleday Lab

At the Department of Oncology-Pathology basic, translational and clinical research and educational activities related to cancer is carried out. Approximately 300 people from over 40 nations are currently working at the department. 37 research groups with various cancer research profiles are involved and we have around 120 PhD students. The Department of Oncology-Pathology is responsible for undergraduate courses in Pathology, Oncology and Forensic Medicine for medical students, as well as for Tumor biology courses for biomedicine students and Pathology courses for opticians.

The Helleday lab is a group of approximately 50 researchers, PhD-Students and postdocs in the Department of Oncology and Pathology at Karolinska Institute, located at SciLife Laboratories in Solna. The overall aim of our research is to improve current cancer treatments by utilizing defects in cancer cells to tailor specific drugs to all types of cancer, with minimal side effects. Our work covers both basic research and translational research and we have a number of interdisciplinary collaborations. The research group is multidisciplinary with expertise in cell biology, biochemistry, pharmaceutical chemistry and pharmacology, with personnel both from academia and industry.

The genomic integrity of mammalian cells is under constant attack from damage arising from endogenous and exogenous sources. Repair of these DNA lesions must be tightly coordinated with other cellular processes including replication and transcription. The importance of fine-tuning transcription with the DNA damage response and repair for cellular survival is underlined by several hereditary disorders associated with predisposition to cancer or premature aging. We aim to characterize the role of new factors in the DDR to gain insights into this interesting connection between transcription and repair. 

Faithful replication of the mammalian genome is essential to maintain genomic stability and prevent the rise of cancer-promoting mutations. Obstacles on the DNA template like unrepaired DNA lesions, bound proteins or secondary structures, as well as chemical agents like Hydroxyurea, can lead to stalling or collapse of replication forks. To activate and restart replication, cells use a specific process called “replication restart”. While a lot is known about replication restart in E. Coli, much less is known about this mechanism in mammalian cells. Our goal is to use the DNA fibre technique and super resolution microscopy to unravel the detailed mechanism of repriming in response to different DNA lesions and study replication restart at the molecular level.

We are seeking a senior researcher who will unravel the coordination of DNA repair, replication and transcription. In addition to the experimental research, the candidate will lead the basic science team within the Helleday laboratory. This role entails mentoring a team of more than five postdoctoral researchers and PhD students on a daily basis, arranging regular team meetings, journal clubs and performing yearly personal development reviews.

Entry requirements
A person is eligible to be appointed Senior Researcher if he or she has been awarded a Degree of Doctor or has achieved the corresponding scientific competence, as well as documented research after publicly defending his or her doctoral thesis corresponding to qualification as Assistant professor.

Applicants must have a doctorate degree in biology and should preferably have at least 5 years of postdoctoral experience. It is an advantage if applicants have research experience from abroad. We prefer applicants to have experience in advanced live cell microscopy methods including FRAP, FLIP, and laser micorirradiation. Profound knowledge in high throughput microscopy is also desirable. In addition the ideal candidate should have experience in immunofluorecence analysis, the DNA fibre technique, and basic molecular techniques such as subcloning, immunoprecipitation and Western Blotting. Furthermore, the candidate must have very good knowledge of the English language and be able to express themselves both orally and in writing in a professional manner. The team leader role requires experience in project management and analytical thinking. Good interpersonal skills and experience in developing new methods is desirable.

Bases of assessment
We stress research expertise when assessing applicants.

Application process
An application must contain the following documents in English: Curriculum vitae, qualifications and description of planned research, presented in accordance with Karolinska Institutet’s qualifications portfolio (http://ki.se/qualificationsportfolio). 

The application is to be submitted through the Varbi recruitment system.  

At Karolinska Institutet a 6-month trial period is required for all permanent appointments.

Type of employment Tillsvidareanställning
Contract type Full time
Number of positions 1
Working hours 100%
City Solna
County Stockholms län
Country Sweden
Reference number 2-1727/2018
  • Camilla Sjögren, 08 524 814 17
Union representative
  • Mimmi Shoshan, SACO, 08 517 754 60
  • Helen Eriksson, OFR, 08 517 762 79
  • Andreas Lundqvist, SACO, 08 517 768 84, andreas.lundqvist@ki.se
Published 27.Mar.2018
Last application date 17.Apr.2018 11:59 PM CET

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