Karolinska Institutet,

The Department of Laboratory Medicine consists of 6 divisions and is active in many areas of Laboratory Medicine. The department is involved in advanced basic scientific, clinical and epidemiological research. The department is one of the largest departments of education at Karolinska Institutet.

We are seeking a highly motivated and ambitious individual to join Division of Pathology, Department of Laboratory Medicine, Flemingsberg, Karolinska Institutet as a Postdoctoral Fellow to develop a 3D in vitro-model for bone biology and to elucidate cell signaling within the bone-remodeling unit.

Dr Tuomas Näreoja  Dr. Tuomas Näreoja was recently provided starting grants from Swedish Research Council and Jane and Aatos Erkko foundation and is currently establishing his research group at the Karolinska Institute. The main research focus of Dr. Näreojas’s group will be to understand signaling in bone remodeling, osteoclast function and inflammatory bone resorption and osteolysis. The group’s long-term aim will be to screen better small molecule drugs for osteoporosis; detect and alleviate inflammatory bone resorption; and to produce bone for applications in regenerative medicine. Dr. Näreoja’s current work focuses on pre-clinical in vitro studies on communication between bone resorbing osteoclasts (OC), osteocytes (OY) inhabiting channels inside bone and bone mineralizing osteoblasts (OB) as they remake bone; and proof-of-concept studies to develop diagnostics of inflammatory bone resorption. 

Project description 
The primary aims of the proposed project are to

  1. further develop our 3D in vitro model (Deguchi et al. 2016) to include OYs, OCs, and OBs, and functionalization (e.g. proteins, NO and pH-probes).
  1. Elucidate and understand the coupling mechanisms in the intertwined signaling between OCs, OBs and OYs in mineralization. While OYs stimulate differentiation of OCs and OBs they also inhibit it by osteoprotegerin and sclerostin, respectively. In our model we can examine the impact of the signals in a sub-cellular resolution enabling us to establish precise molecular mechanisms. The hydrogel allows diffusion of added modulators and bone particle size of sub-wavelength of visible light enables tracking of the induced modulation.
  1. Learn how to modulate balance between mineralization and resorption in an in vitro model replicating the bone-remodeling unit, and to establish quantifiable parameters to describe effects of drug candidates have on the balance of the bone remodeling cycle. In the in vitro model complemented with appropriate growth factors we are able to test osteoinductive drugs in an environment that is able to model their impact on the surrounding signaling network.

Entry requirements
We are seeking a highly talented and enthusiastic researcher with a Ph.D. degree and a background in bone biology and/or cell biology and 3D model development as well as a strong track record in research. Candidates with extensive experience in imaging, stem cell research, cell signaling advanced cell culture protocols and experience with multiparameter flow cytometry and cell sorting are encouraged to apply. Knowledge in bone remodeling, osteoclastogenesis, OB differentiation OB-OC – coupling and osteocyte cultures is preferable. State-of-the-art experience with high-content imaging and analysis is a merit. 

The successful candidate should be self-motivated and able to keep herself/himself informed on developments of the relevant research field, and also, have excellent interpersonal and communication skills, organizational skills, and the ability to work productively and dynamically in a team. Applicant must be able to integrate well into the group and international and multidisciplinary research environment as a whole. Excellent knowledge of spoken and written English is a requirement. The selected candidate will work as part of a team, in close collaboration with graduate and undergraduate students.

PhD-students who have yet to graduate may apply if their thesis defense is scheduled to take place within four months after the application deadline, in which case verification from the Dissertation Committee, or equivalent body, must be submitted with the application.

Type of scholarship
A scholarship for carrying out postdoctoral research can be granted for a maximum of two years within a four year period following the receipt of a doctoral degree or equivalent. This educational scholarship is tax-exempt.

The amount is set for twelve months at a time and is paid out on a monthly basis. In exceptional cases, shorter periods may be acceptable. This is a full-time fixed-term appointment for 12 months, with possibilities for extension depending on scientific progress.

Application process
An application must contain the following documents in English:

1. Motivation letter, including a summary of applicant’s current research and Ph.D. studies (max two A4 pages).

2. Curriculum vitae and qualifications, presented in accordance with Karolinska Institutet’s qualifications portfolio (http://ki.se/meritportfolj )

3. A complete list of publications with description of the applicant’s role in the publication

4. Verifications for crediting of illness, military service, work for labour unions or studentorganisations, parental leave or similar circumstances

5. Verification from the thesis defense committee or the equivalent (only if the thesis defense is scheduled within six months after the application deadline).

6. Names and contact details of two persons who are familiar with the applicant’s recent research and training and are able to provide a letter of reference.

The application is to be submitted on the MyNetwork recruitment system.

Research environment
At Division of Pathology, the groups are part of a highly interactive research environment within bone biology, cancer research and regenerative medicine. The Department of Laboratory Medicine consists of 9 divisions and is active in many areas of Laboratory Medicine. The department is involved in advanced basic scientific, clinical and epidemiological research. The department is one of the largest departments of education at Karolinska Institutet. The department is also tightly connected with clinical researchers and healthcare teams at several departments of the Karolinska University Hospital, which allows for translational research involving clinical material and the potential to bring new therapies from bench to bedside. The facilities at Laboratory Medicine offer state-of-the art cell culture, molecular biology, flow cytometry and microscopy facilities. Our vision is for the department's research to promote human health. This research often leads to new diagnostic methods, clinical practices or to new treatments.

Type of employment Postdoktoralt stipendium
Contract type Full time
Reference number 2-5414/2017
  • Tuomas Näreoja , tuomas.nareoja@ki.se
Union representative
  • Carina Eklund, OFR, 08-585 826 65, carina.eklund@ki.se
  • Emilie Hultin, SACO, 08-585 811 68, emilie.hultin@ki.se
  • Karin Olsson, SEKO, karin.olsson@ki.se
Published 28.Nov.2017
Last application date 18.Dec.2017 11:59 PM CET

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