Karolinska Institutet, Institutionen för neurobiologi, vårdvetenskap och samhälle

The Department of Neurobiology, Care Sciences and Society (NVS) conducts world leading research in aging, dementia, epidemiology and care sciences. This research extends from basic molecular and cell levels to clinical research and societal studies. NVS educates almost a third of KI students at basic and advanced levels. The Department has approximately 450 employees and a revenue 2017 of 470 million SEK. It comprises eight divisions, located at Campus Flemingsberg and Campus Solna.

The research group is located at Karolinska Institutet, campus Huddinge, and at SLU in Uppsala. We are currently five PhD students, two technicians, three post docs, one assistant professor , one senior scientists and one professor. The common theme of our research is the study of protein aggregation, in particular in relation to spider silks and amyloid disease, and the development of efficient recombinant production of aggregation prone proteins and polypeptides (including drug development for lung disease). 

Our group has developed a method for producing aggregation prone polypeptides, e.g. surfactant protein C (SP-C) analogues, in bacterial expression systems (Kronqvist et al. Nat Commun. 2017). The recombinant SP-C analogue is mixed with phospholipids to generate a simple artificial lung surfactant preparation. We will investigate if this lung surfactant preparation can be used to treat acute respiratory distress syndrome in animal models (rabbits and rodents). Lung surfactant spreads rapidly across the mucosal surface and may carry different pharmaceuticals, which makes it possible to develop novel local treatment regimes for lung diseases. Therefore, we will also mix different compounds with our artificial lung surfactant and study the spread and effects in vitro and in vivo.

The successful candidate is expected to have a large interest in experimental animal work, and preferably have a background in lung related research and/or protein biochemistry. The work includes studies of the activity and spread of lung surfactant preparations that may include radioactively labelled molecules in animal trials using mainly rabbits and rats. The job may also include production of proteins in E.coli, subsequent purification, analyses of the produced proteins and production of artificial lung surfactants. The lung surfactant will be analyzed by different methods to determine the level of lipopolysaccharides. In vitro work using culture of different bacterial strains and methods for determining surface activity may also be part of the duties. We work in collaboration with other groups and very interdisciplinary. It is therefore important that the successful candidate can work independently, be creative, have a positive attitude and be collaborative. Previous practical experience and knowledge of work with laboratory animals are required (Felasa C or equivalent). Since the project is a collaboration between KI and SLU the applicant should be prepared to visit both places in the line of work. The position is full time for one year. 

Entry requirements
The applicant is required have previous practical experience and knowledge of work with laboratory animals (Felasa C or equivalent). The successful candidate is expected to have a strong drive to pursue experimental research, and an ambitious and positive attitude. The project is cross-disciplinary and pursued in collaboration with several research groups, and good collaborative skills are therefor necessary. Emphasis will be put on personal skills in the evaluation process. Good ability to read and write in English is required.

Application process
The application is to be submitted on the MyNetwork recruitment system.

Type of employment Visstidsanställning längre än 6 månader
Contract type Full time
City Huddinge
County Stockholms län
Country Sweden
Reference number 2-3846/2017
  • Anna Rising, Senior researcher, anna.rising@ki.se, +46 (0) 70-974 48 88
  • Jan Johansson, Professor,, janne.johansson@ki.se, +46 (0)70-345 70 48
  • Mona Hellstadius, HR, , mona.hellstadius@ki.se, +46 (0) 8-524 868 14
Union representative
  • Agneta Öhlen, SACO, +46 (0) 8-524 837 53, Agneta.Ohlen@ki.se
  • Carina Dahlin, OFR , +46 (0) 8-524 836 92
  • Henry Wölling, SEKO, +46 (0) 8-524 840 80, henry.wolling@ki.se
Published 25.Aug.2017
Last application date 22.Sep.2017 11:59 PM CET

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