Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Molecular Neurobiology

Karolinska Institutet is one of Europe's largest medical universities. It is also Sweden’s largest centre for medical training and research, accounting for 30 per cent of the medical training and 40 per cent of the medical academic research that is conducted nationwide. Karolinska Institutet´s mission is to improve the health of mankind through research, education and information.

The VirusTech core facility
Viruses have emerged as useful tools for efficient delivery of genes to mammalian cells. Thus, it is no wonder that viruses have led the field in gene therapy, as they have evolved to deliver their genes efficiently, in the form of either RNA or DNA into mammalian cells. In research, delivery of genetic information to cellular systems is the backbone of many experiments. The delivery of recombinant genes into neurons is a critically important strategy for understanding the molecular mechanisms underlying brain function, as well as for how these mechanisms go awry in brain disorders. A complementary and equally important strategy is delivery of genetic tools affecting gene expression to over-express, reduce or eliminate a specific RNA or protein.

The core facility specializes in the production and concentration of Lentiviral and Adeno Associated Viruses (AAVs), but it aims to develop and optimize the production of other types of viruses such as Canine Adenovirus, retroviruses, Rhabdo viruses and Herpes simplex viruses. Its primary purpose is to produce and distribute quality viral vectors to researchers within Karolinska Institutet and thereafter investigators world-wide. The core facility is now looking for a lab technician with interest and experience in BSL-2 level work with different types of virus vectors in general and AAVs in particular.

Your role as a lab technician
As you would be directly involved in the production of viruses and mainly working within the BSL-2 lab, your responsibilities would include (but are not limited to):

  • Production of viruses
  • Cataloging, storage and distribution of the produced viruses
  • Taking inventory of the necessary reagents, re-stocking the lab including ordering.
  • Organization of the routine lab work and instruments for daily work
  • Developing and maintaining up-to-date databases of customers and protocols
  • Optimization of existing protocols.
  • Inventory and maintenance of cell lines stored in -80 or liquid nitrogen
  • Documentation of experiments and of viruses produced for customers
  • Feedback to the directors and facility manager on virus production

Qualifications and requirements
Since the facility will handle the production of viruses from different customers from Karolinksa Institutet as well as from other institutions, we are looking for a highly organized, self-motivated person with excellent time management skills who can work effectively in a dynamic and diverse laboratory setting. Experience in biosafety level 2 or higher work for the production and handling of infectious particles, especially Adeno Associated Viruses (AAVs), is meriting. You are structured and organized in your approach to work but flexible and receptive to developmental challenges. You also have good social and interpersonal skills, since you will be working in a multicultural environment, and routinely report progress to the Core facility manager and directors.

Specific requirements:

  • A PhD or Master degree in biological sciences or a relevant field with strong lab work background
  • Documented experience with work in biosafety level 2 laboratories or above.
  • Background in virus biology.
  • Commitment to science and service-oriented with good organizational ability
  • Should possess good written and oral communication skills in English and Swedish.
  • Awareness of the critical aspects of laboratory and chemical safety and the ability to follow procedures and maintain a safe work environment.

Application process
The application is to be submitted on the MyNetwork recruitment system.

Type of employment Visstidsanställning längre än 6 månader
Contract type Full time
City Solna
County Stockholms län
Country Sweden
Reference number 2-1269/2017
  • Jens Hjerling-Leffler, scientist, 08-524 869 74
  • Albert Blanchart Aguado, laboratory coordinator, 08 524 87 293
Union representative
  • Anne Edgren, OFR/S, P, O, 08-616 16 29
  • Biborka Bereczky Veress, SACO, 46 70-173 85 75 , biborka.bveress@ki.se
  • Henry Wölling, SEKO, 08-524 840 80, henry.wolling@ki.se
Published 08.Mar.2017
Last application date 31.Mar.2017 11:59 PM CET

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