Karolinska Institutet, Institutionen för Medicinsk biokemi och biofysik, Translational Medicine and Chemical Biology

A fixed-term (max 6 months) position as Research Assistant is immediately available in the Bienko and Crosetto labs for Quantitative Genome Biology (https://bienkocrosettolabs.org/). The main task of the position will be optimizing GPSeq, a newly developed next-generation sequencing method for studying genome architecture.

Research environment
The Bienko and Crosetto labs are two highly integrated research groups working on genome architecture (Bienko group) and genome fragility (Crosetto group) in healthy and disease conditions. We pioneered BLESS, the first method for direct genome-wide localization of DNA double-strand-breaks DSBs (Crosetto et al., Nature Methods 2013), and recently further advanced the technology by developing BLISS (Yan et al., Nat Commun 2017; Mirzazadeh et al., Meth Mol Biol 2018). We have also developed GPSeq, a new method based on massively parallel sequencing that enables to measure the radial nuclear position of thousands of genomic loci simultaneously (manuscript in preparation). We are applying BLISS and GPSeq, together with a variety of other genome-wide techniques (RNA-seq, HiC), to investigate the relationship between genome architecture and fragility in various contexts.
Our labs are located at the Science for Life Laboratory (SciLifeLab) situated at the Karolinska Institute campus in Stockholm. SciLifeLab is an interdisciplinary center for molecular biosciences with focus on health and environmental research, bringing under the same roof groups from four universities: Karolinska Institutet, KTH Royal Institute of Technology, Stockholm University and Uppsala University. The center features state-of-the-art technology platforms, including next-generation sequencing, high-throughput histology, super-resolution microscopy, proteomics, image analysis, and bioinformatics.

The successful candidate will join an interdisciplinary and dynamic team of international researchers, including clinicians, biologists, biotechnologists, engineers, computer scientists, and physicists. Our mission is to transform the way we understand complex biological phenomena and diseases such as cancer, by integrating next-generation sequencing technologies, single-molecule microscopy methods, and advanced computational tools.

Specific tasks will include:

  1. Cell culture and preparation of samples for GPSeq
  2. Scouting of different assay formats for GPSeq
  3. Validation of GPSeq results using DNA FISH

Entry requirements
The successful candidate shall hold a Master’s Degree in Biomedicine. In addition, she/he should have a clearly documented prior experience with cell culture, basic molecular biology techniques (nucleic acids purification, PCR, in vitro transcription, cDNA synthesis), and DNA and RNA FISH techniques. Prior experience in a lab that routinely performs single-molecule RNA FISH is required. A strong sense of duty and a keen motivation to work in an interdisciplinary and collaborative environment are indispensable.

Application process
An employment application must contain the following documents in English:
  1. A complete curriculum vitae, including date of the thesis defence, title of the thesis, previousacademic positions, academic title, current position, academic distinctions, and committee work
  2. A complete list of publications
  3. A summary of current work (no more than one page)
  4. Verifications for crediting of illness, military service, work for labour unions or student organisations, parental leave or similar circumstances
  5. Verification from the thesis defence committee or the equivalent (only if the thesis defence isscheduled within three months after the application deadline)

Application process
The application is to be submitted on the MyNetwork recruitment system.

At Karolinska Institutet a 6-month trial period is required for all permanent appointments.

Type of employment Temporary position 3-6 months
Contract type Full time
First day of employment upon agreement
Salary Monthly
Working hours 100 %
City Stockholm
County Stockholms län
Country Sweden
Reference number 2-5375/2018
  • Nicola Crosetto, +46 (0)73 986 53 79
Union representative
  • Biborka Bereczky Veress, SACO, 46 70-173 85 75 , biborka.bveress@ki.se
  • Anne Edgren, OFR/S, P, O, 08-524 87570
  • Henry Wölling, SEKO, henry.wolling@ki.se
Published 05.Oct.2018
Last application date 15.Oct.2018 11:59 PM CET

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