Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Chemical Biology and Genome Engineering

HTGE is a national SciLifeLab facility based at the Department of Medical Biochemistry and Biophysics at Solna campus. HTGE offers pooled genetic screening using CRISPR/Cas to the Swedish research community on a fee-for-service, non-profit basis. Customer projects come from all fields of molecular cell biology, and HTGE thus is an interesting and challenging work environment with great opportunities for personal development. HTGE was established in early 2017, and after a successful first year, is now looking to expand its team.

Job description.
The successful applicant will perform genetic screens using CRISPR technology for customers, and will have regular customer contact. Daily duties will consist of relatively large-scale mammalian cell culture, lentiviral work, and molecular biology such as cloning and library preparation for next generation sequencing. Depending on qualifications, an involvement in HTGE’s own rsearch and development is possible. Responsibilities will also include general lab management, which is not anticipated to take more than 10% of the full time.

Required skills.
You hold a PhD degree in the life sciences, and have a proven track record of being able to carry out complex scientific experiments. You are an expert in culturing mammalian cells, and you have extensive experience in molecular biology techniques such as DNA isolation, PCR, cloning, etc. You have, or are willing to acquire, basic skills in bioinformatics. You are motivated and enjoy expanding your skill set. You are highly organized, meticulous, self-motivated and you manage your time efficiently. You have excellent communication skills, and are fluent in English.

Advantageous skills.
An understanding of the principles of pooled genetic screening, experience in working with lentivirus, and familiarity with next generation sequencing methods are advantageous. Expertise in FACS analyses, previous experience with CRISPR/Cas9 in either precision genome engineering and/or in high-throughput screening, as well as experience with primary cell cultures are additional merits.


Your application must include the following documents in English:

  • A cover letter, clearly stating why you are interested in this position and how your skills match what we are looking for.
  • A full curriculum vitae (please leave no gaps) including a full list of publications (if applicable).
  • Two to three letters of reference should be sent directly to bernhard.schmierer@ki.se by the persons who provide the reference.

For further information www.scilifelab.se/htge
The application is to be submitted on the MyNetwork recruitment system

Type of employment Temporary position longer than 6 months
Contract type Full time
First day of employment upon agreement
Salary Monthly
Working hours 100 %
City Stockholm
County Stockholms län
Country Sweden
Reference number 2-995/2018
  • Bernhard Schmierer, +46 8 524 800 00 or bernhard.schmierer@ki.se
Union representative
  • Anne Edgren, OFR/S, P, O, 08-616 16 29
  • Biborka Bereczky Veress, SACO, 46 70-173 85 75 , biborka.bveress@ki.se
  • Henry Wölling, SEKO, henry.wolling@ki.se
Published 15.Feb.2018
Last application date 20.Mar.2018 11:59 PM CET

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