Institutionen för medicin, Solna, Enheten för reumatologi

The Department of Medicine, Solna, is one of the largest departments at Karolinska Institutet. The department consists of several units that are separated into different research groups. The core activities of the department are research, doctoral education and undergraduate education for medical and biomedical students. The research has three main profiles: allergy, cardiovascular medicine and inflammatory diseases.

Division
The Department of Medicine is since March 2015 one of the partners of a new SGC and IMI project focused on the discovery and validation of novel targets in inflammatory diseases (ULTRA-DD, Unrestricted Leveraging of Targets for Research Advancement and Drug Discovery). The position will initially be placed within the research group of Professor Per-Johan Jakobsson, Rheumatology unit, Department of Medicine, Karolinska Institutet, Solna.

Project
This large scale project is a collaboration between the Karolinska Institutet, University of Oxford (UK) and the ETH in Zürich (Switzerland), with funding from the European Union Innovative Medicines Initiative and pharmaceutical company partners. ULTRA-DD is conducted as an integrated project with the Structural Genomics Consortium (SGC) in Toronto (Canada) and Oxford (UK) (www.thesgc.org). The ULTRA-DD organization based at Karolinska Institutet comprises funding for around 10 staff members, and is expected to grow as additional funding becomes available. Overall, ULTRA-DD has activities e.g. in structural and chemical biology, antibody production and characterization as well as functional studies of human proteins in disease relevant assays.

The post holder will report to Louise Berg, group leader of the Stockholm section of Workpackage 6 with responsibility of developing patient-derived cell assays for screening of pro-drugs. Scientific and clinical advice will be obtained from a local management advisory committee consisting of experts in the field, at the Department of Medicine and at Karolinska University Hospital. Ultimately, the post holder reports to Michael Sundström, Scientific Director at the SGC and ULTRA-DD.

The research project is focused on screening of pro-drugs in patient-derived cellular assays, using blood and tissue from patients with autoimmune diseases and inflammatory bowel diseases (IBD), partly funded by and in collaboration with Takeda Pharmaceuticals. Focus will be on differentiating gut-derived stem cells into gut organoids, and on immune based assays (ELISA-like assays). Our aim is to establish advanced functional and translational assays using such organoids, biopsies and cells from peripheral blood of patients, to participate in early drug development in collaboration with pharmaceutical industry partners. The chemical probes used in these studies (high quality chemical modulators of protein function) mainly affect epigenetic regulation and phosphorylation dependent signaling, and initial results have already been obtained in primary cell assays from patients with autoimmune inflammatory diseases such as SLE, myositis and systemic sclerosis.



Duties
To culture organoids derived from stem cells isolated from the intestinal wall of patients with IBD, under the supervision of Carlos Villaescusa, Department of Molecular Medicine and Surgery, CMM. To run ELISA-based assays (ELISA, mesoscale, luminex) on cell- and tissue-derived culture supernatants in the screening platforms run within the group.

Entry requirements
We are seeking an experienced and dedicated person, who will be based at our laboratories at the Center for Molecular Medicine (CMM), employed by the Department of Medicine, Karolinska Institutet. We are seeking a candidate with experience of working in a research laboratory, who communicates well, is well structured and takes responsibility to produce well documented and clearly communicated high quality scientific data.

Required:

  • Experience in human cell culture
  • Experience in ELISA-based techniques
  • Experience in electronic lab journal documentation
  • Good knowledge of handling data analysis in excel
  • Experience in handling laboratory equipment coupled to computers
  • Good communicative skills in English (written and oral)

Desirable:

  • Experience in handling human tissues

Application process
The application is to be submitted in the Varbi recruitment system.

Type of employment Temporary position longer than 6 months
Contract type Full time
First day of employment accordingly too agreement
Salary individual salary
City Solna
County Stockholms län
Country Sweden
Reference number 2-6001/2017
Contact
  • Louise Berg, louise.berg@ki.se
Union representative
  • Lars Ährlund-Richter, SACO, 08 - 5177 34 50, lars.ahrlund@ki.se
  • Nasrin Bavand, 08-517 723 64
Published 20.Dec.2017
Last application date 14.Jan.2018 11:59 PM CET

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